Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source
نویسندگان
چکیده
منابع مشابه
JBO LETTERS Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source
A 12-fs broadband 100-nm source is used to combine multiphoton microscopy MPM and optical coherence tomography OCT in a single platform. An ultrafast Ti:sapphire laser simultaneously provides short pulses necessary for efficient MPM excitation and the broad bandwidth required for high-resolution OCT. Using 0.3m microspheres and a 63 , 0.95 numerical aperture objective, we demonstrate that MPM a...
متن کاملCombined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source.
A 12-fs broadband (100-nm) source is used to combine multiphoton microscopy (MPM) and optical coherence tomography (OCT) in a single platform. An ultrafast Ti:sapphire laser simultaneously provides short pulses necessary for efficient MPM excitation and the broad bandwidth required for high-resolution OCT. Using 0.3-microm microspheres and a 63x, 0.95 numerical aperture objective, we demonstrat...
متن کاملCharacterizing refractive index and thickness of biological tissues using combined multiphoton microscopy and optical coherence tomography
We present a noninvasive method for characterizing the refractive index (RI) and thickness distribution in biological tissues using a combined multiphoton microscopy (MPM) and optical coherence tomography (OCT) system. Tissue layers are distinguished by the MPM and OCT images, and the RI and thickness of each layer are determined by analyzing the co-registered MPM and OCT images. The precision ...
متن کاملCombined two-photon microscopy and angiographic optical coherence tomography.
A combined two-photon microscopy (TPM) and angiographic optical coherence tomography (OCT) is developed, which can provide molecular, cellular, structural, and vascular information of tissue specimens in vivo. This combined system is implemented by adding an OCT vasculature visualization method to the previous combined TPM and OCT, and then is applied to in vivo tissue imaging. Two animal model...
متن کاملImaging subcellular scattering contrast by using combined optical coherence and multiphoton microscopy.
The structural origin of scattering contrast from single cells is examined by using a combined optical coherence and multiphoton microscope based on a 12 fs Ti:sapphire source and a 0.95 NA objective. High-resolution coherence-gated scattering images from single cells are coregistered and compared with two-photon-excited fluorescence images. Scattering contrast is observed from mitochondria, pl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2006
ISSN: 1083-3668
DOI: 10.1117/1.2193428